Human Immunodeficiency Virus (HIV) is an RNA virus. It is a lentivirus, a retrovirus member family which causes Acquired Immunodeficiency Syndrome (AIDS). An early event in every retroviral multiplication is the integration of the viral double-stranded DNA genome into the host chromosome. The integration is facilitated by the activation of integrase.The goal of this research is to obtain the HI…
Background:Previous studies in Indonesia with a limited sample of HIV-1 subtypes revealed that HIV-1 subtype distribution and mode of transmission differed between the regions. This analysis aimed to describe subtypes and mode of transmission of HIV-1 in wider area in Indonesia. Methods:This analysis used a part of HIV/AIDS cross sectional study in seven provinces in Indonesia in 2011 among …
Background: The quantitative assays are important tests in the management of patients with HIV-1/AIDS. The important step in developing the assay is the availability of the standard HIV-1 RNA. For this purpose, we optimized in vitro HIV-1 RNA transcription to produce the standard HIV-1 RNA. Methods: The HIV-1 DNA was amplified from pNL43 by PCR using a primer pair that was specific for conse…