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Gene S characterization of Hantavirus species Seoul virus isolated from Rattu…
Hantavirus lives and reproduces in the body of rodents. Rattus norvegicuswas one found in the Kepulauan Seribu islands of Indonesia. Hantavirus species Seoul virus (SEOV) is a negative single chain RNA viruses included in the family Bunyaviridae. It has a few specific genes, especially genes S that can be developed for a diagnostic test. The aim of this study was to ascertain the character of g…
- Edition
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- ISBN/ISSN
- 2087-7021
- Collation
- 6p
- Series Title
- -
- Call Number
- Health Science Journal of Indonesia, 5 (1) :1-6
4. Potential Cross-Reactivity of Severe Acute Respiratory Syndrome-Associated…
Aim: to evaluate the specificity of the SARS-CoV N protein-based IgG ELISA assay for detection of immunoglobulin G (IgG) in plasma samples obtained from HIV-1 positive and HIV-1 negative intravenous drug users (IDUs). Methods: the SARS-Co V N gene was cloned into pQE-80L vector, and the constructs were transformed into Escherichia coli BL21. The 6 x His-tagged N protein was expressed by induci…
- Edition
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- ISBN/ISSN
- 0125-9326
- Collation
- 6p
- Series Title
- -
- Call Number
- Acta Medica Indonesiana: The Indonesian Journal of
In vitro transcription of HIV-1 RNA for standard RNA
Background: The quantitative assays are important tests in the management of patients with HIV-1/AIDS. The important step in developing the assay is the availability of the standard HIV-1 RNA. For this purpose, we optimized in vitro HIV-1 RNA transcription to produce the standard HIV-1 RNA. Methods: The HIV-1 DNA was amplified from pNL43 by PCR using a primer pair that was specific for conse…
- Edition
- -
- ISBN/ISSN
- 0853-1773
- Collation
- 5p
- Series Title
- -
- Call Number
- medical journal of indonesia, 20 (3) 2011 : 185-18
Rapid Detection of Ethambutol-resistant Mycobacterium tuberculosis Directly f…
Aim: to develop an assay system of a radioisotope (32P)-based PCR dot-blot hybridization technique and evaluation of the assay directly for TB sputum samples to detect mutation at codon 306 of embB gene of Mycobacterium tuberculosis related with ethambutol (EMB) resistance. Methods: one hundred and sixteen of sputum samples were used in this study. Bacterial genome in sputum samples was extr…
- Edition
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- ISBN/ISSN
- 0125-9326
- Collation
- 4p.
- Series Title
- -
- Call Number
- Actamedica Indonesia, 43(1)2011: 34-38
In vitro transcription of HIV-1 RNA for standard RNA
Background: The quantitative assays are important tests in the management of patients with HIV-1/AIDS. The important step in developing the assay is the availability of the standard HIV-1 RNA. For this purpose, we optimized in vitro HIV-1 RNA transcription to produce the standard HIV-1 RNA. Methods: The HIV-1 DNA was amplified from pNL43 by PCR using a primer pair that was specific for conse…
- Edition
- -
- ISBN/ISSN
- 0853-1773
- Collation
- 4p.
- Series Title
- -
- Call Number
- Medical Journal of Indonesia:20(3)2011:185-189
Development of Multiplex-PCR Assay for Rapid Detection of Candida spp.
Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identifi cation of Candida spp. is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specifi city. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp. Methods F…
- Edition
- -
- ISBN/ISSN
- O853-1773
- Collation
- 4p.
- Series Title
- -
- Call Number
- Medical Journal of Indonesia:19(2)2010:83-87
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