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Analisis Gen Haemagglutinin pada Virus Campak Liar (Analysis of Haemagglutini…
Penyakit Campak disebabkan oleh virus campak yang termasuk genus Morbilivirus dan Family Paramyxoviridae. Penyakit campak masih menjadi masalah kesehatan karena masih ditemukan Kejadian Luar Biasa (KLB) di Indonesia. Salah satu penyebab terjadinya KLB tersebut diduga sebagai akibat perbedaan antigenesitas antara strain vaksin yang digunakan dengan strain virus campak liar yang beredar di Indon…
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- ISBN/ISSN
- 0853-9987
- Collation
- -
- Series Title
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- Call Number
- Media Penelitian dan Pengembangan Kesehatan, 25 (1
4. Potential Cross-Reactivity of Severe Acute Respiratory Syndrome-Associated…
Aim: to evaluate the specificity of the SARS-CoV N protein-based IgG ELISA assay for detection of immunoglobulin G (IgG) in plasma samples obtained from HIV-1 positive and HIV-1 negative intravenous drug users (IDUs). Methods: the SARS-Co V N gene was cloned into pQE-80L vector, and the constructs were transformed into Escherichia coli BL21. The 6 x His-tagged N protein was expressed by induci…
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- ISBN/ISSN
- 0125-9326
- Collation
- 6p
- Series Title
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- Call Number
- Acta Medica Indonesiana: The Indonesian Journal of
In vitro transcription of HIV-1 RNA for standard RNA
Background: The quantitative assays are important tests in the management of patients with HIV-1/AIDS. The important step in developing the assay is the availability of the standard HIV-1 RNA. For this purpose, we optimized in vitro HIV-1 RNA transcription to produce the standard HIV-1 RNA. Methods: The HIV-1 DNA was amplified from pNL43 by PCR using a primer pair that was specific for conse…
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- ISBN/ISSN
- 0853-1773
- Collation
- 5p
- Series Title
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- Call Number
- medical journal of indonesia, 20 (3) 2011 : 185-18
In vitro transcription of HIV-1 RNA for standard RNA
Background: The quantitative assays are important tests in the management of patients with HIV-1/AIDS. The important step in developing the assay is the availability of the standard HIV-1 RNA. For this purpose, we optimized in vitro HIV-1 RNA transcription to produce the standard HIV-1 RNA. Methods: The HIV-1 DNA was amplified from pNL43 by PCR using a primer pair that was specific for conse…
- Edition
- -
- ISBN/ISSN
- 0853-1773
- Collation
- 4p.
- Series Title
- -
- Call Number
- Medical Journal of Indonesia:20(3)2011:185-189
Development of Multiplex-PCR Assay for Rapid Detection of Candida spp.
Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identifi cation of Candida spp. is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specifi city. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp. Methods F…
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- ISBN/ISSN
- O853-1773
- Collation
- 4p.
- Series Title
- -
- Call Number
- Medical Journal of Indonesia:19(2)2010:83-87
Deteksi Antibodi Anti H5N1 dengan Uji hambatan
Snce July 2005 until May 2008, Indonesia has reported 133 confirmed cases with case fatality proportion 81%, 54% had history of direct contact with poultry with poultry (chicken). It is important to detect antibody anti H5N1 among high risk population like poultry collector workers in DKI Jakarta. Antibody anti H5N1 were tested by Hemaglutination Inhibition (HI) assay using A/Ck/West Java /67/0…
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- ISBN/ISSN
- 0853-7704
- Collation
- 7p.
- Series Title
- -
- Call Number
- Jurnal Respirologi Indonesia, 29(1) 11-17
Deteksi Antibodi Anti H5N1 dengan Uji hambatan
Snce July 2005 until May 2008, Indonesia has reported 133 confirmed cases with case fatality proportion 81%, 54% had history of direct contact with poultry with poultry (chicken). It is important to detect antibody anti H5N1 among high risk population like poultry collector workers in DKI Jakarta. Antibody anti H5N1 were tested by Hemaglutination Inhibition (HI) assay using A/Ck/West Java /67/0…
- Edition
- -
- ISBN/ISSN
- 0853-7704
- Collation
- 7p.
- Series Title
- -
- Call Number
- Jurnal Respirologi Indonesia, 29(1) 11-17
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