In monitoring the treatment of malaria with Dihydroartemisinin-piperaquine (DHP), microscopic cross check and Polymerase Chain Reaction (PCR) performed to validate the results of laboratory examinations in the field. This study used finger prick samples from subjects with a diagnosis of malaria in monitoring the treatment of malaria with DHP in Kalimantan and Sulawesi. Samples taken at day 0, b…
Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identifi cation of Candida spp. is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specifi city. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp. Methods F…
The isolation and characterization of chromosomal genes and bacterial plasmids are important for strain improvement to produce most valuable compounds on industrial scale. The isolation of the neutral protease (npr) fragment from Bacillus megaterium was carried out by polymerase chain reaction (PCR). The PCR experiments were done setting up of variation for oligonucleotide primers, annealing te…
The clinical diagnosis of thalassemia syndrome is not difficult to establish with relatively simple Investigations, but molecular study is needed to determine the genotype and for the prenatal diagnosis. In the molecular study, the DNA should be first amplifed and among the amplification techniques polymerase chain reaction is the simplest and the most rapid one. The procedure can in vitro ampl…
Staphylococcus aureus (S. aureus) sebagai penyebab terpenting infeksi luka pascaoperasi dapat ditransmisikan melalui tangan dan hidung perawat. Tujuan penelitian ini untuk mengetahui kesamaan strain Staphylococcus aureus pada perawat di ruang bedah dan infeksi luka pascaoperasi. Kesamaan strain S. aureus dideteksi dengan metode konvensional, kemudian perbedaan antara strain S. aureus ditentukan…
Tuberculosis (TB) remans an important health problem in Indonesia. Diagnosis of pulmonary tuberculosis reqires sputum examination and isolation of M. tuberculosis from a culture specimen. The limitation of acid-fast bacilli (AFB) micropic has relatively low sensitivity and solid media culture of M. tuberculosis required for long time (4-6 weeks) indentifying M. tuberculosis Approximately 30-60%…