Medical Biotechnology, Biopharmaceutics, Forensic Science and Bioinformatics

This book covers a range of topics on exploiting Nigeria’s mega biodiversity for food security and health; DNA forensic science and its applications; medical biotechnology and biopharmaceutics; medicinal and underutilized plants; impact and mitigation of antibiotic resistance; bioinformatics applications; medical insect biotechnology; etc. The book will be useful reference material for the sc…

Petunjuk Teknis Pencegahan dan Pengendalian Penyakit Monkeypox

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x. 66p. : bibl., ; illus.; 21 cm.

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WC 584 CHI 2019

Pedoman Pelaksanaan Pengabdian Kepada Masyarakat Tematik : Posdaya Berbasis M…

Edition

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ISBN/ISSN

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Collation

xiv. ; 59p. ; illus.; 21cm.

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Gene S characterization of Hantavirus species Seoul virus isolated from Rattu…

Hantavirus lives and reproduces in the body of rodents. Rattus norvegicuswas one found in the Kepulauan Seribu islands of Indonesia. Hantavirus species Seoul virus (SEOV) is a negative single chain RNA viruses included in the family Bunyaviridae. It has a few specific genes, especially genes S that can be developed for a diagnostic test. The aim of this study was to ascertain the character of g…

Analisis Gen Haemagglutinin pada Virus Campak Liar (Analysis of Haemagglutini…

Penyakit Campak disebabkan oleh virus campak yang termasuk genus Morbilivirus dan Family Paramyxoviridae. Penyakit campak masih menjadi masalah kesehatan karena masih ditemukan Kejadian Luar Biasa (KLB) di Indonesia. Salah satu penyebab terjadinya KLB tersebut diduga sebagai akibat perbedaan antigenesitas antara strain vaksin yang digunakan dengan strain virus campak liar yang beredar di Indon…

Acalypha indica Linn root extract improved hippocampal cell viability and inc…

Background: This study was done to determine the effect of root extract of Acalypha indica Linn (akar kucing) in protecting neuron viability ofthe rat hippocampus on tissue culture in hypoxic condition. Methods: This is an experimental study of in vitro prirnary cell culture of hippocampus of Sprague Dowley aduh rat. The cultures were group into control (C) and exposure to root extract of Aca…

4. Potential Cross-Reactivity of Severe Acute Respiratory Syndrome-Associated…

Aim: to evaluate the specificity of the SARS-CoV N protein-based IgG ELISA assay for detection of immunoglobulin G (IgG) in plasma samples obtained from HIV-1 positive and HIV-1 negative intravenous drug users (IDUs). Methods: the SARS-Co V N gene was cloned into pQE-80L vector, and the constructs were transformed into Escherichia coli BL21. The 6 x His-tagged N protein was expressed by induci…

In vitro transcription of HIV-1 RNA for standard RNA

Background: The quantitative assays are important tests in the management of patients with HIV-1/AIDS. The important step in developing the assay is the availability of the standard HIV-1 RNA. For this purpose, we optimized in vitro HIV-1 RNA transcription to produce the standard HIV-1 RNA. Methods: The HIV-1 DNA was amplified from pNL43 by PCR using a primer pair that was specific for conse…

Induksi Sel Somatik Menjadi Sel Punca Pluripoten

Human embryonic stem cell has been used extensively in research; it has certain advantagecompared to animal stem cell, but application in clinical setting is ethically problematik.Nuclear reprogramming attempt to produce pluripotent cells made a breakhrough by the discovery of induced pluripotent stem cells from adult somatik cells. Transfection of 4 transcription factors Oct3/4.c-Myc . Sox2 an…

In vitro transcription of HIV-1 RNA for standard RNA

Background: The quantitative assays are important tests in the management of patients with HIV-1/AIDS. The important step in developing the assay is the availability of the standard HIV-1 RNA. For this purpose, we optimized in vitro HIV-1 RNA transcription to produce the standard HIV-1 RNA. Methods: The HIV-1 DNA was amplified from pNL43 by PCR using a primer pair that was specific for conse…