Introduction: Diagnostic tuberculosis using acid fast bacilli (AFB) microscopy and conventional Lowenstein Jensen (U) culture remain the cornerstone but the sensitivity of these traditional methods is quite low, especially in the samples containing small number of organism. There is a need for rapid, sensitive and accurate detection of these organisms in clinical specimens to hasten the adminis…
Background: Mycobacterium tuberculosis antigen38 is a potent serodiagnostic agent containing two M. tuberculosisspecific B-cell epitopes. The high price of imported diagnostic agents hinders realization of fast clinical TB diagnosis in developing countries. Therefore, we produced recombinant antigen38 (recAg38M) from M. tuberculosis local strain, which might be used to produce economical tuberc…
Aim: to develop an assay system of a radioisotope (32P)-based PCR dot-blot hybridization technique and evaluation of the assay directly for TB sputum samples to detect mutation at codon 306 of embB gene of Mycobacterium tuberculosis related with ethambutol (EMB) resistance. Methods: one hundred and sixteen of sputum samples were used in this study. Bacterial genome in sputum samples was extr…