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Identifikasi Single Nucleotide Polymorphism (SNP) Gen pvmdr1 pada Penderita Malaria Vivaks di Minahasa Tenggara (Sulawesi Utara)

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Parasite resistance to antimalarial drugs is an obstacle to malaria elimination. In Plasmodium vivax, up to now, a marker to distinguish between resistant and susceptible is no available yet. Identify Single Nucleotide Polymorphisms (SNP) in P. vivax for multidrug resistance (pvmdr1)is potential approach due to pvmdr1 gene is orthologous to the pfmdr1 of P.falciparum, have been related to multidrug resistance.

The purpose of this study was to identify SNPs/mutations on pvmdr1 gene of malaria vivax patients who came to the Primary Healthe Centers, Touluaan and Tombatu Minahasa Tenggara (North Sulawesi).Blood samples and slide of blood smears were collected from patients who infected with P.vivax or mixed infection of P.vivax and P.falciparum. After the species were cross checked by certified microscopist then confirmed by PCR, SNP identification were performed by sequencing technique. Only 83 of 99 recruited subjects were included inclution criteria. Sequensing result showed that 59 of 83 subjects were analysed to identify the SNP. We found 5 nonsynonymous SNPs, namely at the point G698S, M908L, Y976F, L1076F, and K1261E.

Key words: Plasmodium vivax, Single Nucletide Polymorphism, Gen pvmdr1, Mutation

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Detail Information

Series Title

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Call Number

Jurnal Biotek Medisiana Indonesia, 3 (2) : 49-57

Publisher

Jakarta : Pusat Biomedis dan Teknologi Dasar Kesehatan., 2014

Collation

8p

Language

ISBN/ISSN

2301-5810

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Media Type

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Edition

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Subject(s)

MUTATION
PLASMODIUM VIVAX
POLYMORPHISM, SINGLE NUCLEOTIDE

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