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Pengaruh Iodium dan Selenium terhadap Jumlah Sel Spermatogonium dan Struktur Histologis Tubulus Seminiferus Testis Tikus Wistar Hipotiroid (Iodine and Selenium Effect on Spermatogonia Cell Numbers and Histologist Structure of Seminiferous Tubules Testis Hypothyroid Wistar Rats)

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Thyroid hormones are proven to have a direct effect on sexual development and reproductive function. Hypothyroidism in men cause decreased libido, impotence, and oligospermie. Thyroid disorders associated with abnormal testicular morphology and function. Selenium was closely related to male fertility. Glutathione peroxidase 4 (GPx4) was first known as the antioxidant enzymes is selenoenzyme which is dominant in testis allegedly important for spermatogenesis.

The aim of this study was to evaluate spermatogonia cell numbers and the histological structure of seminiferous tubules of hypothyroid rats as a result of the intervention with iodine and selenium.

An experimental study with post-test only control group design. Fifty hypothyroidism male Wistar rats induced by Propylthiouracil (PTU) for four week were divided into three groups through simple random sampling. Group I treated with iodine, group II treated with iodine + selenium and group III is control group. Sampling to determine groups by randomization. Blood sample was taken and then Thyroid Stimulating Hormone (TSH) blood level was measured using an Enzyme-Linked Immunosorbent Assay (ELISA). Whereas, spermatogonia cell numbers and the histological structure of seminiferous tubules was measured using Hematoxylin Eosin (HE) histologist. Anova test was used to compare the data obtained from treated and control groups of TSH blood level and spermatogonia cell numbers. Data of seminiferous tubules histological structure were analyzed by comparing between groups.

TSH blood level in group I (3.5 ± 4.9 μIU/mL), group II (1.9 ± 1.5 μIU/mL, and group III (13.5 ± 8.3 μIU/mL) was significantly difference with value (p=0.000). However, spermatogonia cell numbers on group I (66.6 ± 18.1), group II (57.4 ± 3.3), on group III (53.6 ± 5.3) was not significantly difference with value (p=0.204). Observation of the seminiferous tubules in group I and II showed that spermatogenic cell structure appeared more clearly, with more full spermatogenic structures, narrower tubular lumen contains lots of sperm. Observations in group III showed abnormal seminiferous tubules, irregular arrangement of spermatogenic cells with wider lumen contains few sperm.

Iodine and selenium had no effect on the average number of spermatogonia cells and affected the histological structure of the seminiferous tubules in wistar rats testis.

Keywords: hypothyroid, iodine, selenium, spermatogenesis, TSH

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Series Title

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Call Number

Media Gizi Mikro Indonesia, 6 (1) : 1-10

Publisher

Magelang : Balai Litbang GAKI., 2014

Collation

10p

Language

ISBN/ISSN

2086-5198

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Edition

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Subject(s)

selenium
spermatogenesis
iodine

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