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Metalloproteinase-9 gene variants and risk for hypertension among ethnic Javanese (Variasi genetik gets penyandi metalloproteinase-9 dan risiko hipertensi pada etnis Jawa)

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ABSTRACT

BACKGROUND

Hypertension is associated with endothelial-dependent vasodilation disorders, due to reduced nitric oxide (NO) availability and excessive angiotensin II (ANG¬II) activation. The objective of this study was to determine the association between matrix metallopeptidase 9 (MMP-9) gene polymorphism and hypertension in ethnic Javanese in the 40-80 year age group.

METHODS
This was a case-control study on 50 PROLANIS patients of family doctors meeting the inclusion criteria and 50 controls without hypertension. Subjects were hypertensive patients with constant systolic arterial pressure of >140 mmHg and diastolic arterial pressure of >90 mmHg, confirmed in three successive measurements The observed parameters were degree of MMP-9 polymorphism, and NO and ANG-II levels. Matrix metallopeptidase 9 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using the Smal restriction enzyme. MMP-9 polymorphisms were indicated by variation in band patterns. Degree of polymorphism in cases and controls were compared with NO and ANG-II levels in both groups. Data analysis was done using independent t-test.

RESULTS

The heterozygous (3 band) to normal (2 band) MMP-9 genotype ratio was 3:1 in hypertensives, but balanced in controls. In hypertensives, heterozygous GA and homozygous AA genotype frequencies were respectively 3.198 and 1.548 times higher than that of the GG genotype (p-0.008 and r0.726). There was a statistically significant differences of NO and Ang-11 levels between cases and controls (1)=0.000 and r0.000; respectively).

CONCLUSION
Matrix metallopeptidase 9 gene polymorphisms in hypertensive ethnic Javanese are associated with NO and angiotensin II levels.

Keywords: Hypertension, ethnic Javanese, MMP-9 gene polymorphism, adults

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UNIVERSITAS MEDICINA, 33 (3) 2014 : 213-220

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Jakarta : UNIVERSA MEDICINA/FK UNIVERSITAS TRISAKTI., 2014

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8p

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1907-3062

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