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Selaginella active fractions induce apoptosis on T47D breast cancer cell

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Apoptosis is an important target on anticancer mechanism. The purpose of this research is to investigate apoptosis induction of Selaginella plana Hieron active fractions on T47D cells. Absolute ethanol was used to extract Selaginella /Diana powders. Ethanolic extract was dilluted by methanol:water (4:1) and then fractionated by hexane (S Hex), methylene chloride (S_MTC), ethyl acetate (S_EA), and buthanol (S_BuOH). The proliferation of T47D cell line was detected by SRB (Sulforhodamine B) assay which was measured at a wavelength of 515nm. Flowcytometry analysis to determine apoptosis was examined by Propidium Iodide (PI) and Annexin V assay using T47D breast cancer cell line. The result showed that the IC50 value of S Hex, S MTC, S_EA, and S_BuOH on T47D cells were 107 pg/mL, 4 pg/mL, 6 pg/mL, and 17 pg/mL respectively. The active fractions (S_MTC and S_EA) at its IC50 concentration significantly (P

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Series Title

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Call Number

Majalah Farmasi Indonesia, 23 (1) 2012 : 48-53

Publisher

Yogyakarta : Faculty of Pharmacy Universitas Gajah Mada Yogyakarta., 2012

Collation

6p

Language

ISBN/ISSN

0126-1037

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Media Type

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Carrier Type

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Edition

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Subject(s)

breast neoplasms
APOPTOSIS

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