Pengaruh Perlakuan PGV-1, PGV-0 dan Kurkumin terhadap Protein yang Terlibat dalam Siklus Sel Fase G2-M dan Apoptosis pada Sel Kanker Payudara T47D (The Effect of PGV-1, PGV0 and Curcumin on Protein Involve in G2-M Phase of Cell Cycle and Apoptosis on T47D Breast Cancer Cell Line) | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Pengaruh Perlakuan PGV-1, PGV-0 dan Kurkumin terhadap Protein yang Terlibat dalam Siklus Sel Fase G2-M dan Apoptosis pada Sel Kanker Payudara T47D (The Effect of PGV-1, PGV0 and Curcumin on Protein Involve in G2-M Phase of Cell Cycle and Apoptosis on T47D Breast Cancer Cell Line)

Muhammad Da'i - Personal Name; Edy Meiyanto - Personal Name; Masashi Kawaichi - Personal Name; Umar Hanggara Jennie - Personal Name; Supardjan AM - Personal Name;

Previous experiments show ed that curcumin analogue (PGV-1) inhibited breast cancer cell (T47D) growth at G2-M phase and induced cell apoptosis. This experiment was conducted to investigate the molecular effect of another curcumin analogue, PGV-0 and curcumin on the cell cycle progression and apoptosis as compared to the PGV-I. Flowcytometric method was conducted to analyze the effect of PGV- 1(2,5 µM), PGV-0(5,0 µM) and curcumin (10,0 µM) on the cell distribution ofvarious phase of T47D cell cycle. Western blot was also conducted to observe the effect ofthose compounds on proteins that involved in cell cycle (i.e. p21 and Cdc-2) and apoptosis (Caspase-3/7/9).
The results showed that PGV-1, PGV-0 and curcumin induced hyperploidy phenomenon on T47D cell. PGV-1inhibited the cell cycle specificallyon G2-M phase. Molecular observation showed that PGV-1 and PGV-0 were able to increase the expressiion of p21 protein and the Cdc-2 proteins, whilst curcumin was able to activate the Cdc-2 protein. The compounds have ability to induce apoptosis on T47D cell via Caspase3/7 activation. In conclusion, PGV-1, PGV-0 and curcumin inhibited the T47D cell cycle progression and induced cell apoptosis.
Keywords: curcumin, PGV-O, PGV-1, cell cycle, apoptosis, T47D cell.

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Location name is not set Jurnal Ilmu Kefarmasian Indonesia, 10 (2) 2012 : 9

A0003342

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Series Title: -
Call Number: Jurnal Ilmu Kefarmasian Indonesia, 10 (2) 2012 : 9
Publisher: Jakarta : Jurnal Ilmu Kefarmasian Indonesia FF Universitas Pancasila., 2012
Collation: 12p
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ISBN/ISSN: 1693-1831
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