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Acalypha indica Linn root extract improved hippocampal cell viability and increased Brain-derived Neurotrophic Factor (BDNF) in hypoxic condition

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Background: This study was done to determine the effect of root extract of Acalypha indica Linn (akar kucing) in protecting neuron viability ofthe rat hippocampus on tissue culture in hypoxic condition.
Methods: This is an experimental study of in vitro prirnary cell culture of hippocampus of Sprague Dowley aduh rat. The cultures were group into control (C) and exposure to root extract of Acalypha indica Linn with dose of 10 mglmL, 15 mg/mL, and 20 mg/mL for 72 hours. The cultures were then exposed to hypoxic gas (5% oxygen, 5% carbondioxide, nitrogen balance) for 24 hours. After that, relative cell viability was measured by 3-[ 4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), cell proliferation by 5-bromo2 '-deoxy-uridine (BrdU), and Brain-derived Neurotrophic Factor (BDNF) levels by BDNF ELISA kit.
Results: The result showed MTT viability(C: 99.7%, A indica L 10: 326.3%,A indica L 15:411.7%, A indicaL20: 445.9%), BrdU absorbance (C: 0.07,A indica L 10: 0.10,A indica L 15: 0.12, A indica L 20: 0.13) ofthe exposured hippocampal cell were significantly higherthan the control group (p < 0.01) accompany by increased level of BDNF (C: 11.3 pg/mL,A indica L20: 12.5 pg/mL, A indica L 15: 23.1 pg/mL, A indica L 20: 18.1 pg/mL).
Conclusion: The root extract of Acalypha indica Linn is ab Ie to improve rat hippocampal cell viability and endogenous BDNF levels in hypoxic condition.
Keywords: BDNF, hippocampal cell viability, hypoxia, root of Acalypha indica Linn

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Series Title

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Call Number

Medical Journal Indonesia, 21 (3) 2012 : 141-146

Publisher

Jakarta : FK universitas indonesia., 2012

Collation

6p

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ISBN/ISSN

2252-8083

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Subject(s)

HIPPOCAMPUS
HIPOXIA
BRAIN-DERIVED NEUROTROPHIC FACTOR

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