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Alpa-Lipoic Acid Ca Not Preven Effect on Cell Viability, Collagen Synthesis Inhibition, Collagen Degradation Induction in Ultraviolet AIrradiated Human Firoblast Cell

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Ultraviolet A (UVA)irradiation on human skin can generate free radical and stimulate matrix metalloproteinase produktion resultingin collagen degradation and transforming growth factor beta (TGF-β) inhibition. It causes synthesis collagen inhibition and induces cell death. Α-Lipoic acid (ALA) is an universal antioxidant and a powerful scavenger of free radicals. In this study we investigated the effect of ALA on viability collagen synthesis and degradation in UVA irradiated human fibroblasts. Normal human skin fibroblasta cell culture were irradiated with UVA for three times with each dose of 3000 mJ/cm₂ UVA. Α-Lipoic acid in various concentration was added to the culture following UVA irradiation and incubated for 48 hours. The cell viability was determined by MTT assay while collagen synthesis and degradation were determined Sirius red binding assay. The difference of cell viability and collagen synthesis and degradation between fibroblasts cell after and without UVA irradiation were analyzed using paired-ttest with 95% confidence interval (p

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Journal of the Medical Sciences:43(2)2011:87-93

Publisher

Yogyakarta : Faculty of Medicine Gajah Mada University., 2011

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6p.

Language

English

ISBN/ISSN

0126-1312

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