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Active Compounds Isolated From red Betel (Piper Crocatum Ruiz & Pav) Leaves active Agains Streptococcus Mutans Through its Inhibition Effect on Glucosyltransferase Activity

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Dental caries is a major problem of dental health in Indonesia. Streptococcus mutans is a bacteria that can cause caries. In the recent time, the agents used to eliminate the caries is less efective due to S. Mutans resistance to those alternative agents dental caries is needed. Red betel (piper crocatum Ruiz & Pav) is a promising natural material to treat dental caries. The P. Croatum Ruiz & Pav leaves contain secondary metabolites such as essential oil, flavonoid, alkaloid, and phenolic compounds that active against S. Mutans involved in caries formation. This study aimed to evaluate the activity of secondary metabolites isolated from red betel leaves against S. Mutans and its inhibition effects on the glucocyltransferase (GTF) activity. The growth inhibition test of S. Mutans was carried out with liquid dilution test and diffusion test. Cultures of S. Mutans were treated with assential oil, alkaloid, flavonoid, phenolic compounds isolated from P. Crocatum Ruiz & Pav in various concentration and incubated at 37⁰Cin anaerob condition. Providone iodine was used as positive control and aquadest as negative control. The inhibition test of GTF activity was performed by measuring the levels of formed fructose that was analyzed by Somogyinelson method. The data was analyzed using one way ANOVA with a 95% confidence interval. The results showed that essential oil and alkohol had lowest MIC (Minimal inhibitory) Concentration) compared with the other active compounds (5000 µg/mL) but higher than povione iodine as positive control (2500 µg/mL). The essential oil exhibited similar inhibition zone diameter compared with pevidone iodine, either at low or high concentration (p>0.05). The essential oil had similar inhibition activity to the glucosytransferase compared with povidone (p>0.05). In conclusion, the essential oil of red betel leaves had the strongest against the S. Mutans growth trough its inhibition effect on GTF activity.

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Journal of the Medical Sciences:42(2)2011:71-78

Publisher

Yogyakarta : Faculy of Medicine Gajah Mada University., 2011

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7p.

Language

English

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0126-1312

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