Optimal Temperature for Normal Human Skin Fibroblast Proliferation and Glucose Uptake, an in Vitro Study | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Optimal Temperature for Normal Human Skin Fibroblast Proliferation and Glucose Uptake, an in Vitro Study

Devi Artami Susetiati - Personal Name; Laily Noor Qomariah - Personal Name; Sunardi Radiono - Personal Name; Yohanes Widod Wirohadidjojo - Personal Name;

Wrinkles is caused by a decreased by a decrease in collagen synthesis an incease in collagen degradation. Collagen synthesis depends on fibroblast proliferation. Collagen synthesis needs glucose, so that collagen synthesis may be expressed by the increase in glucose uptake. Skin rejuvenation with heating may increase the collagen synthesis. The effect of short-term heating and optimal temperature on fibroblast proliferation and glucose uptake has not yet been defined. This effect of short-term heating and optimal temperature on fibroblast proliferation and glucose uptake has not yet been defined. This study was aimed to determine the optimal temperature of short-term heating for normal human skin fibroblasts proliferation and glucose uptake. A simple experimental study was conducted on 3rd passage subculture of normal human skin fibroblasts culture, isolated from 2 patients, Normal human skin fibroblasts with complete DMEN were classified into 5 groups, and then heated for a minute with thermocycler-machine at 36⁰C, 46⁰C, 55⁰C, 66⁰C, and 72⁰C. Those cells were incubated for 7 days in complete DMEN and subsequently fibroblasts proliferation was measured by MTT-assay. Glucose uptake was measured by glucometer Medisafe-terumo. The differences I glucose uptake and fibroblasts proliferation were analyzed with one-way Anova. Optimal temperature for fibroblasts proliferation was 46⁰C, and 66⁰C for collagen symthesis. Skin rejuvenation based on heating could be performed at two different temperatures, each cycle consisted of 66⁰C on first heating, and days later, at 46⁰C on second heating.

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Location name is not set Berkala Ilmu Kedokteran, 41(3): 118-121

A00002366

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Series Title: -
Call Number: Berkala Ilmu Kedokteran, 41(3): 118-121
Publisher: Yogyakarta : FK. Universitas Gadjah Mada., 2009
Collation: 4p.
Language: English
ISBN/ISSN: 0126-1312
Classification: -
Content Type: -
Media Type: -
Carrier Type: -
Edition: -
Subject(s): skin
FIBIOBLASTE
GLUKOSE
Specific Detail Info: -
Statement of Responsibility: -

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