ABSTRACT Introduction: Spilanthes acmefla or which already knowby society as Legetan. is one of Indone¬sian medicinal plants In previous in vitro study, it was reported that 70% ethanol extract, buihanol and water.fractMn from this plant have increased alkaline phaspharase that brown as marker of bone formation. Aim. The objective of this in vivo study was to analysis the anti osteoporosis …