ABSTRACT An in vitro experimental research was conducted between October 2013 and January 2014. Neem lea were extracted in ethanol or hexane at various concentrations. A macrodilution test with 48-hour incubation time was done in triplicate on 8 groups of samples. These comprised the neem leaf ethanol extract (NLEE) at 0.5, 1.0, and 2.0 g/dL, neem leaf hexane extrac…
The culture media commonly used for isolation are imported and expensive. Many organic materials are naturally decomposed from complex organic compounds to simple ones by microbes. Based on this principles, this study wants to make isolation media with ripe coconut waste-water as based substance, so it can be considered as economical culture media. The method was laboratoric experimental by iso…