Background: Piper retrofactum Vahl has been used as traditional medicine for a long time. Objective: to compare: (1) piperine alkaloid and other alkaloid of cabe Jawa from Madura and Lamongan, by Thin Layer Chromatography (TLC) method,(2) the effective dose (ED50) of anticonvulsive of infusum extract and mefenemic acid by shock seizure (MES) and metrazole seizure (MET) in Mice, (3) ED50 of anal…