Positive control of Plasmodium spp. is needed in identification of malaria species using Polymerase Chain Reaction (PCR) method. The limitedness of malaria positive control encourages us to develop the cloning method to multiply Plasmodium spp. DNA particularly P.vivax, P. malariae, and P. ovale that are still unable to be cultured continuously. DNA product of single round and nested PCR were u…