Frozen-thawed human spermatozoa are routinely used for many assisted reproduction program. However, cryopreserved spermatozoa was reported to yield lower pregnancy rates compared to fresh semen in both intra uterine inseminations and in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) due to the reduction of sperm motility and viability induced by cryopreservation procedures. Thi…