Abstract: Isolation and identification of secondary metabolites from root of Jatropha gossypifolia, and also its biological activity test toward microbes and turnor cells have been conducted. isolation was carried out by using vacuum Iiqiud chromatography (VLC) method (adsorbent: silica gel, eluent: mixture of n-hexane, ethyl acetate, chloroform, and methanol). The isolate molecular structure w…