Based on national health research 2010, the coverage of basic immunization on West Kalimantan were 52,1%. It’s causes concern of outbreaks some immunizable prevented diseases in West Kalimantan . One of them is diphtheria. It was reported incidence of diphtheria suspect in Dalam Bugis, Pontianak Timur, Kota Pontianak, West Kalimantan in April 2013. A suspect of 12 years old girl was reported …
Diagnosis of diphtheria caused by Corynebacterium diphtheriaeshould be done immediately since delay of therapy may cause 20-fold increase rate of death. One method of rapid diagnostic to identify diphtheria is by using polymerase chain reaction (PCR). The fundamental issue of this method depends on the DNA, either its quality or quantity. The simple DNA extraction method, which is using mechani…