A study was conducted in the Vector Control Research Station laboratory in Salatiga and in ponds belongs to inhabitants of Sukutukan village. Wulanggitang Sub District,
A study was carried out in Vector Control Research Station (VCRS) laboratory in Salatiga. This study was conducted to determine the pathogenecity of Bacillus thuringiensis and Bacillus sp. isolation from various soil habitat. The test were detected by Chilcott Wigley's method (1988). Result show a high sensitivity of larvae toward B. thuringiensis and Bacillus sp. Four isolates of B. thuringi…
The isolation and characterization of chromosomal genes and bacterial plasmids are important for strain improvement to produce most valuable compounds on industrial scale. The isolation of the neutral protease (npr) fragment from Bacillus megaterium was carried out by polymerase chain reaction (PCR). The PCR experiments were done setting up of variation for oligonucleotide primers, annealing te…
Bacillus thuringiensis H-14 was tested as follows : Laboratory trials revealed a LC50 at 3.2027 ppm and 1.4998 ppm and LC90 at 12.2521 ppm and 7.3356 ppm at exposures of respectively 24 and 48 hours. Small scale trials in ricefield plots in Hilisimaetano village showed a reduction rate of respectively 85.54 percent-89.54 percent, 88.36 percent-94.61 percent and 88.61 percent-94.55 percent at do…