NS1 protein is a multifunction protein that plays key role of pathogenesis and virulence of avians influenza virus H5N1. The amino acid substitution at the position P42S, D92E, F103I, M106I and 5 amino acid deletion at the position 80 to 84 in NS1 protein reported increasing virulence of avians influenza virus H5N1. Several studies showed avians influenza virus H5N1 in Indonesia has dynamic cha…