Embryonic Stem Cells (ESC) are pluripotent stem cells which has the ablility to self renew and differentiate into form all cells in the body. Inner Cell Mass (ICM) as a source of ESC that can be obtained from blastocyst stage of embryos. Isolation can be done by several methods such as mechanical, enzymatic and immunosurgery. This study aimed to observe the effectiveness of usage immunosurg…
Induced Pluripotent Stemcell (iPS) are adult cells which the genetic information in the nucleus of those cells being reprogrammed (reprogram) by inserting exogenous pluripotential genes. The exogenous gene transduction is using vectors, such as lentivirus, retrovirus, or adenovirus, which suppressed the gene expression of the original cells, so they will express the transduced exogenous gene. V…
Embryonic Stem Cells (ESCs) is a one of source for pluripotent stem cell that capable to differentiate into various cell types. This has opened up opportunities utilization of embryos as a source of stem cells. However, the use of embryos as objects of research still has ethical constraints. Currently parthenogenetic embryo in the blastocyst stage is considered to be one of the alternative …
Background: Stem cell reprogramming has been used as a source of pluripotent cells due to controversy associated with utilization of embryonic stem cells. However, some of reprogramming methods used involve risky genetic modifications. The objective of this review is to discuss a safer reprogramming method using non-oncogenic recombinant proteins. Methods: This article was made with prior sea…
The study of stem cell therapy in the world has grown rapidly in the last few decades, especially in cardiovascular disease such as acute myocardial infarction, end stage coronary heart disease and cardiomyopathy. In the future, stem cell therapy is con side red as the ultimate limit (the final frontier) treatment of various diseases. Therefore, the development of stem cell therapy is expected …