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Comparative Study on Residual Effects of Bacillus thuringiensis H-14 and Bacillus sphaericus H-5a5b Against Aedes aegypti Larvae in Some Types of Water Container (Studi Koparatif Efek Residual Bacillus thuringiensis H-14 dan Bacillus sphaericus H-5a5b terhadap Larva Aedes aegypti pada Beberapa Tipe Tempat Penampung Air)
Bacillus thuringiensis H-14 and Bacillus sphaericus H-5a5b are microbial agents showing high potency for vector control. They are highly specific to target insect, and do not produce any adverse environmental impact. Such agents would be very promising agents for vector control, especially vector of dengue haemorrhagic fever in Indonesia. The present studies aimed at observing the residual effects of B. thuringiensis H-14 (VCRC B17) and B. sphaericus H-5a5b (VCRC B42) on the larvae of Aedes aegypti in some types of water container. Two steps of the studies were carried out under laboratory conditions. First step were bioassays to determine of VCRC B17 and VCRC B42 potencies. Second steps were residual effect testings to determine of the residual activities of both VCRC B17 and VCRC B42 in the comented, clay, and plastic containers. Bioassays of VCRC B17 and VCRC B42 on Ae. aegypti larvae shawed that the Lethal Concentrations 50 percent were 117.9 ug/l and 790.6 ug/l respectivelly. Residual effect of VCRC B17 on Ae. aegypty larvae at the concentrations ranging from 1 to 125 mg per litres, in the cemented container was 34 to 91 days, in the clay container was 28 to 49 days, and in the plastic container was 21 to 66 days. Similar studies of VCRC B42 on the same species of mosquito larvae and the same concentration range the residual effects were 3 to 26 days, 3 to 14 days, and 16 days respectively in the cemented, clay, and plastic containers.
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