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Effect of Caffeine through the Quality of Human Sperms In-Vitro (Studi Kefein terhadap Kualitas Spermatozoa Manusia In-Vitro)

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The motility of ejaculated human spermatozoa and its ability to penetrate cervical mucus represent an important parameters confirm the quality of human semen. Poor quality of semen could not fulfill this criteria, especially for artifial insemination with husband's semen. The addition of caffeine has been noted to increase life-span and forward progression of especially poor quality spermatozoa. Whether this treatment of caffeine actually results in enhancement of the quality of human spermatozoa, represent an interesting investigation. The purpose of the present study was to investigate the effects of caffeine on the mortility of ejaculated human spermatozoa and ability to penetrate the artificial mucus of polyacrylamide gel. Twenty ejaculated human semen specimens of normazoospermia group was obtained from different individuals. The donors were all male members of infertile couples who came to the infertility clinic at the Department of Biology, University of Indonesia School of Medicine. The specimens were divided into two groups of 500 ul each. To each of control sample, 100 ul of Tyrode solution was added. And to experimental samples, 100 ul Tyrode containing 36 mM of caffeine were added. The results show that cafeine increased the motility, vecolity, and the artificial mucus penetration of ejaculated human spermatozoa.

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Jurnal Kedokteran Yarsi,3(1)1995:46-58

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: ., 1995

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spermatozoa
caffein

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