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Current Diagnosis on Parasitic Infection (Diagnosis Mutakhir Infeksi Parasit)

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Traditionally, the diagnosis of parasitic infection is generally made by examining blood, urine, faecal and other specimens. However the results of this traditional diagnosis are still not satisfying until present. To overcome the problem other diagnostic techniques are being developed to yield better results. Recently, the immunodiagnosis and a DNA probe are used to diagnose filariasis, whereas to detect microfilaria in small quantities the polymerase chain reaction (PCR) is used. In entomology, the ELISA using monoclonal antibody against sporozoit is done in order to find/to determine various malaria vector species in various malarial endemic areas. This technique can also be developed to determine sporozoite index which is important for malaria epidemiology. For malaria immunological assays the competitive antibody binding assays and the immunoradiometric assays (IRMA) have been used to detect parasite antigen whereas soluble antigens in the plasma can be detected with monoclonal antibody (MAb) against Plamodium falciparum exoantigen (molecular weight 50 Kd). Another developed method for the diagnosis of malarial infection by detecting parasite DNA in blood is to use the quantitative buffy coat (QBC). The technique can also be used for the detection of microfilaria and other hemoparasites (e.g. Trypanosoma) in blood.

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Majalah Kedokteran Indonesia,45(2)1995:94-98

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: ., 1995

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parasitic diseases

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