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3-Acetyl -2,5,7-Trihydroxy-1,4-Naphtalenedione, An Antimicrobial Metabolite From The Culture Of Endophytic Fungus Coelomycetes Tcbp4 From Tinospora crispa

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Isolation, identification and testing of antimicrobial activity of secondary metabolites of endophytic fungal culture TCBP4 isolated from bratawali (Tinospora crispa) has been performed. The fungus TCBP4 was cultivated in Potato Dextrose Broth (PDB) for 1 month, media and fungi extracted with ethyl acetate.

The extract was separated and purified by several chromatographic techniques, from which 9 fractions were obtained. Fraction 3e was purified again and was obtained 6 fractions (3e1-3e6). Fractions 3e3-3e6 were tested against bacteria isolates Staphylococcus aureus Bacillus subtilis,Eschericia coli and yeast isolate Candida albicans by microdillution method.

Antimicrobial activity test result showed that fractions 3e3-3e5 had better antibacterial activity compared to chloramphenicol as commercial antibiotic. It was indicated by MIC value of the fractions was lower (8 ug/ml) compared with the antibiotic chloramphenicol (16 ug/ml). Fraction 3e3 had better antifungal activity compared to commercial antifungal nystatin and cabisidin against C. albicans. GC-MS analysis showed that the chemical constituent of 3e5 fraction was identified as 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione

Key words : Tinospora crispa, endophytic fungi, isolation, identification, antimicrobial

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Series Title

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Call Number

Media Litbangkes, 23 (3) 2013 : 95-101

Publisher

Jakarta : Sekretariat Badan Litbangkes., 2013

Collation

7p

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ISBN/ISSN

0853-9987

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Subject(s)

fungi
METABBOLISM

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