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Perkembangan Koloni Primer Embryonic Stem Cell (ESC) Mencit Pasca Vitrifikasi Inner Cell Mass (ICM)

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Application or the basic research of the stem cells is continously conducted to explore their potential, particularly the use of stem cells in health. Cryopreservation technology is needed to maintain availability of stem cell.

This study was aimed to observe the development of murine embryonic stem cell (ESC) primary colonies post-vitrified inner cell mass (ICM). Inner cell mass (ICM) was derived from blastocysts of murine embryos of female Swiss webster mice using pregnant mare’s serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Inner Cell Mass (ICM) was isolated from blastocysts by immunosurgery. Inner Cell Mass (ICM) were divided into two groups: the control and vitrified ICM. Both groups were cultured and observed on the ICM attachment (attachment rate/AR), the primary formation of ESC colonies (primary colony/PC) and diameter of primary colony ESC which grouped into small, medium and large.

The results showed that AR and medium diameter’s group of primary colony ESC were lower (p0.05). This study shows that vitrification for cryopreserving ICM can be used as an alternative source of ESC.

Key words: blastocyst, inner cell mass, ICM, vitrification

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Series Title

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Call Number

Buletin Penelitian Kesehatan, 41 (3) : 171-178

Publisher

Jakarta : Sekretariat Balitbangkes., 2013

Collation

8p

Language

ISBN/ISSN

0125-9695

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Edition

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Subject(s)

EMBRYONIC STEM CELL

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