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Deteksi Brugia malayi pada Armigeres subalbatus dan Culex quinquefasciatus yang Diinfeksikan Darah Penderita Filariasis Dengan Metode PCR

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Pemayungdistricts, Batanghari regency of Jambi province classified as filariasis endemic areas in Jambi province since the Mf rate reached 1.5% in 2011.

A study was conducted to identify Brugia malayi on experimentally infected Ar. subalbatus and Cx. quinquefasciatus. An experimental study was performed with completely randomized design and six repetitions. Standard of treatment in this study was time (hours) that selected for mosquitoes to bite the patients with filariasis (experimental infection). Selected time is at 9.00 a.m, 5.00 p.m, 9.00 p.m, and at 1.00 a.m.

The results showed that filarial L3 larvae did not found on Ar. subalbatus and Cx. quinquefasciatus mosquitoes during surgery at day 11th to 13th after infection. Density of microfilariae in the blood of humans as a source of infection was 17 microfilariae per 20 micro liter blood. Otherwise, after detection by PCR, our study found positive B.malayi on Cx. quinquefasciatus thorax and proboscis. It indicates that Cx. quinquefasciatusas potential vector of B.malayi filariasis compared to Ar. subalbatus.

Keywords: PCR, filariasis, Armigeres subalbatus, Culex quinquefasciatus, Brugia malayi

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Series Title

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Call Number

Aspirator, 6 (2) : 35-42

Publisher

Ciamis : Loka Litbang P2B2 Ciamis., 2014

Collation

8p

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ISBN/ISSN

2085-4102

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Subject(s)

filariasis
Brugia malayi

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