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Penerapan Diagnostik Laboratorium pada Kasus Tersangka Positif Difteri pada Kejadian Luar Biasa di Kota Pontianak, Kalimantan Barat

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Based on national health research 2010, the coverage of basic immunization on West Kalimantan were 52,1%. It’s causes concern of outbreaks some immunizable prevented diseases in West Kalimantan . One of them is diphtheria. It was reported incidence of diphtheria suspect in Dalam Bugis, Pontianak Timur, Kota Pontianak, West Kalimantan in April 2013. A suspect of 12 years old girl was reported with a clinical diagnosis of diphtheria.

Confirmation of positive suspect of diphtheria outbreaks occurred in West Kalimantan. A suspected case of diphtheriae and her mother were taken throat swabs on April 4, 2013, then she was retaken throat swabs and also 29 of her classmate as a contacts of case on April 24th 2013. Microscopic examination by staining Albert. Culture and isolation using blood agar and blood agar telurit. Suspect colonies are followed by biochemical tests using the API Coryne commercial product. Toxigenic test performed by polymerase chain reaction. A suspected case throat swab was taken on 4 April 2013 shows a microscopic results found difteroid form.

Then the results of culture, isolation and biochemical have possibility > 89.5% of bacteria Corynebacterium diphtheriae type mitis. Toxigenic test showed that positif toxigenic bacteria which characterized by amplification gene tox (dtx) along 248 bp. Meanwhile her mother is a negative culture results C.diphtheriae. On 24th April 2013 the suspected case was re-taken of throat swab including 29 contacts from her classmate which showing negative culture result of C.diphtheriae. The suspected case was infected of C.diphtheriae type mitis toxigenic. It’s cases makes an outbreak in West Kalimantan.

Key words : Corynebacterium diphtheriae, Culture and isolation, Biochemical test

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Series Title

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Call Number

Jurnal Biotek Medisiana Indonesia, 3 (1) : 31-36

Publisher

Jakarta : Pusat Biomedis dan Teknologi Dasar Kesehatan., 2014

Collation

6p

Language

ISBN/ISSN

2301-5810

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Edition

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Subject(s)

culture
LABORATORIES
CORYNEBACTERIUM DIPHTHERIAE

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