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Produksi Embryonic Stem Cell (Esc) Line dari Blastosis Mencit dengan Metode Immunosurgery

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Embryonic Stem Cells (ESC) are pluripotent stem cells which has the ablility to self renew and differentiate into form all cells in the body. Inner Cell Mass (ICM) as a source of ESC that can be obtained from blastocyst stage of embryos. Isolation can be done by several methods such as mechanical, enzymatic and immunosurgery.

This study aimed to observe the effectiveness of usage immunosurgery method to obtain the ICM from the blastocyst stage of embryo. Blastocyst stage of embryos were obtained from a strain of Swiss Webster female mice that had been stimulated using pregnant mare 's gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Inner Cell Mass (ICM) were cultured and observed of the attachment level (attachment rate/AR), the rate of primary colony formation (primary colony/PC) and the morphology of ESC line.

The results showed that the ICM were isolated using methods immunosurgery have AR and PC respectively 78,57% and 71,00%. Embryonic Stem Cell (ESC) can differentiate after several passages by forming embryoid body (EB). This study shows that immunosurgery is an effective method for producing ESC line from mice embryos of blatocyst stage.

Key words: Blastocyst, Inner cell mass, ICM, ESC

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Series Title

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Call Number

Jurnal Biotek Medisiana Indonesia, 3 (1) 2014 : 23

Publisher

Jakarta : Pusat Biomedis dan Teknologi Dasar Kesehatan., 2014

Collation

10p

Language

ISBN/ISSN

2301-5810

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Edition

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Subject(s)

blastocyst
EMBRYONIC STEM CELL

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