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Kajian Induced Pluripotent Stem cell (iPS) (Harapan dan Tantangan)

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Induced Pluripotent Stemcell (iPS) are adult cells which the genetic information in the nucleus of those cells being reprogrammed (reprogram) by inserting exogenous pluripotential genes. The exogenous gene transduction is using vectors, such as lentivirus, retrovirus, or adenovirus, which suppressed the gene expression of the original cells, so they will express the transduced exogenous gene. Viral vectors are then used to reprogramming and producing iPS clones that are pluripotent. iPS derived from adult cells of patient with certain diseases will be used as a tool to study the mechanisms of those specific diseases and the effects of selected drugs against the diseases.

Several previous studies have shown that iPS clones developed from specific genetic disease have its original genotype and retain the character of the response to the drug that similar as the original adult cells. Opportunities for the utilization of autologous iPS cell therapy in the future is wide open as expected iPS transplant will not be rejected when transplanted back to the patient. Behind all its potential, iPS production is still facing some problems to be applicable clinically.

The use of viruses as vectors may cause problems due to virus gene sequences may be integrated into the genome of the DNA donor cell, thereby causing mutations of the iPS clones. Several subsequent studies have succeeded in replacing the use of viruses as vectors, but the level of efficiency obtained is still very low. Another problem that arises is that epigenetic changes may occur in iPS cultures. Many advanced research related to iPS may be developed in Indonesia and is necessary to improve the production efficiency of iPS and solve iPS clones epigenetic changes problems in the future.

Keywords: iPS, pluripotency, transduction, transfection.

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Detail Information

Series Title

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Call Number

Buletin Penelitian Kesehatan, 42 (1) : 71-78

Publisher

Jakarta : Sekretariat Balitbangkes., 2014

Collation

8p

Language

ISBN/ISSN

0125-9695

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

STEM CELL

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