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Kandungan Komponen Fenolat, Kadar Fenolat Total, dan Aktivitas Antioksidan Madu dari Beberapa Daerah di Jawa dan Sumatera (Study of Phenolic Compounts, Total Phenolic, and Antioxidant Activities of Monofloral Honeys from Some Areas in Java and Sumatera)

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Many diseases resulted from degenerative processess which can be inhibited by antioxidant systems. Honey is one of food with antioxidant activity.

This study aims to investigate antioxidant activities of several types of monofloral honey from Java and Sumatera.

A laboratory experimental study, conducted on 4 types of floral honeys: coffee, palm trees, cottonwoods and rambutan. Determination of phenolic compounds was performed with High Performance Liquid Chromatography (HPLC) and measurement of total phenolic contents performed with Folin-Ciocalteu’s reagent. Antioxidant activity was conducted in two ways, those were by 2.2-diphenyl-1-picrylhydrasyl (DPPH) free radical scavenging method and linoleic acid peroxidation method using butylated hydroxytoluene(BHT) as a standard. Data were analyzed using Analysis of varian (Anova) and continued with Duncan’s multiple range test (DMRT).

Four types of Javanese and Sumateranese honeys contained some phenolic compounds. Those are chlorogenic acid, cafeic acid, ρ-coumaric acid, ferulic acid, pinobanksin, quercetin, luteolin, pinocembrin and chrysin while the dominant fenolic compound varies between honeys. Total phenolic contents from four types of honey were between 2.000 to 4.400 ppm. The highest phenolic content was in honey from cottonwoods, but the best antioxidant activity was found in honey from coffee. Antioxidant activities were found in honey which come from the following order: coffee, cottonwoods, palm trees, and rambutan honey. Antioxidant activities did not correlated with total phenolic content.

Javanese and Sumateranese honey contained nine active substances that varies in total phenolic contents. The best antioxidant activity was found in coffee honey, and this activity did not correlated with its total phenolic contents.

Keywords: antioxidant, monofloral honey, phenolic

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Detail Information

Series Title

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Call Number

Media Gizi Mikro Indonesia, 6 (1) : 11-24

Publisher

Magelang : Balai Litbang GAKI., 2014

Collation

14p

Language

ISBN/ISSN

2086-5198

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Media Type

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Edition

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Subject(s)

HONEY

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