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Analisis Gen Penyandi Schistosoma japonicum Gluthation s Transferase (SJ26GST) di Dataran Tinggi Lindu, Sulawesi Tengah Indonesia

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Schistosomiasis is only found at Napu and Lindu highland, Central Sulawesi in Indonesia. Schistosomiasis still as a public health problem, with its prevalence increase every year. The large scale by mass drug treatment using praziquantel has done to reduce the prevalence since 1980.

To look for the possibility evidence of the development of resistance in S. japonicum to praziquantel in endemic areas by analysis of Schistosoma japonicum Gluthation S Transferase (Sj26gst) Coding Gene. Moleculer laboratory study was conducted to analyse the sequences of S. japonicumgluthation s transferase gene (Sj26GST). DNA was extracted from adult S. japonicum using isopropanol. Sj26GST gene was amplified used gradient PCR.

The PCR result then run with electrophoresis and viewed using gel-doc. The Sj26GST band was cut and purified using Gene Aid Purification kitand amplified by PCR cycle sequencing, and the product was sequenced using Abi PRISM 310 Genetic analyser. The gene sequences of Sj26GST analysis showed that the homology was very high between isolate from Indonesia and several isolates from China that known still susceptible to praziquantel..

The results indicate that there was no evidence for reduced susceptibility of S. japonicum to praziquantel despite its extensive use in the endemic areas of Napu and Lindu for more than 20 years

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Detail Information

Series Title

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Call Number

Buletin Penelitian Kesehatan, 42 (4) : 231-236

Publisher

Jakarta : Sekretariat Balitbangkes., 2014

Collation

6p

Language

ISBN/ISSN

0125-9695

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

SCHISTOSOMA JAPONICUM
DRUG RESISTANCE
SCHISTOSOMIASIS
PRAZIQUANTEL

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