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Variasi Genetik Oncomelania hupensis lindoensis dengan Metode Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) di Sulawesi Tengah Tahun 2011

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Schistosomiasis is a parasitic zoonotic disease caused by Schistosoma japonicum.In Indonesia, S. japonicumis only found in three endemic areas in the highlands of Central Sulawesi in Lindu valley, Napu and Bada with the snailOncomelania hupensis lindoensisas intermediate host.

The aim of the study was to examine variation genetic of Oncomelania hupensis lindoensis using RAPD PCR. The research divided into two phases which were field research for collecting Oncomelania hupensis lindoensisas samples and laboratory research, consisted of DNA isolation, quantification DNA, dilution DNA, RAPD PCR, electrophoresis and visualisation electrophoresis. Variation genetic of O.h.lindoensis from the schistosomiasis endemic areas was in the form of polymorphism levels, which were respectively 54.55%, 50%, 45.45%, 22.22%, 16.67%, and 14.29%.

The results of this research showed a difference in genetic variation in the form of polymorphism of Oncomelania hupensis lindoensisfrom Lindu, Napu, Bada, highlands.

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Call Number

Buletin Penelitian Kesehatan, 42(2): 79-84

Publisher

Jakarta : Sekretariat Badan Litbangkes., 2014

Collation

6p

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ISBN/ISSN

0125-9695

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Subject(s)

dna
POLYMERASE CHAIN REACTION.

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