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Efek Catechins Teh Hijau terhadap Ekspresi ROR α dan C/EBP-α pada Kultur Primer Preadiposit (Effect of Green Tea Catechins on ROR α and C/EBP-α Expression on Preadipocyte Primary Culture)

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Obesity is o major risk factor for most of chronic diseases with high mortality. Catechins, the active constituent of green tea has been regarded beneficial as an anti-obesity. This study was aimed to investigate the effect of catechins on the positive and negative regulators of lipo genesis and odipogenesis; ROR-a and C/EBP-α.

This study was performed in primary cultured of preodipocyte divided into 4 treatment groups: (1)control, (2) catechins 5 µM, (3) catechins 10 µM and (4) with catechins 30 µM. Two days post confluence, cell were induce to differentiate into adipocyte with IBMX, DEX and insulin. On days 3 of differentiation, cells were treated with catechins for 24 hour Cells then harvested and measurement of ROR-α and C/EBP-α were performed using ELISA and immunocytochemistry.

Result show that catechins significantly reduced the expression of C/EBP-a by 20,24% don 26,43%. it was identified that found that ROR-a expression increased, but not reach statistical significance. These results suggested that cotechins at leostin part inhibit adipogenesis through C/E8P-α.

Keywords: Adipogenesis, catechins, C/EBP-α, ROR-α

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Jurnal Kedokteran Brawijaya 28 (3) 2014 : 131-136

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Malang : Jurnal Kedokteran Brawijaya., 2014

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6p

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0216-9347

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