Text

Uji Anti Plasmodium Ekstrak Batang Tinospora crispa terhadap Plasmodium fakiparum Galur 3D7 secara in Vitro (Antiplasmodial Test of Tinospora crispa Stem Extract against Plasmodium falciparum 307 Strain In Vitro) ; Fitri LE

---

ABSTRACT
This study aimed to prove the existence of anti-plasmodiat activity from Tinospora crispa crispa) stem extracts and to determine the 1C, values as well as the best T. crispa stem extract concentration in inhibiting the growth of P fakiparum 307 strain in vitro. The degree of parasitemia was observed by counting the number of infected red blood cells in Giemsa stained blood films under a light microscope with a magnification of 2000x. The Plasmodium DNA concentration was measured using flow cytornetryc with Propidium Iodide (PI) staining. Analysis of fourier transform infrared (FM) spectrophotometer showed that the methanol extracts of T. crispa stem contained tinocrisposide compound.

From the quantitative test results of Thin Layer Chromatography (TLC), it was obtained 0,22 % alkaloids that might contain compounds of berberine and palm atine. One way ANOVA statistical analysis showed that the degree of parasitemia and the concentration parasite DNA of treatment group of dose of 2,0mg/ml was significantly lower compared to the control group after 48 hours (p=0,001) and 72 hours (p=0,001) of incubation. The T crispastem methanol extract has anti-plasmodium activity, with IC50values between 0,27 mg/ml and 0,29mg/ml, and the effective dose to inhibit the growth ofP fakiparum 3D7 strain is 2,0 mg/ml with reducing parasitemia degree by 47,12% and 56,83% after 72 hours of incubation. From this study shown the methanol extract of T. cris pastern was able to reduce the parasitemia degree of P folciparum 3D7 strain in vitro and could to bee potential candidate forantimalarias.

Keywords: Antiplasmodium, methanol extract, Tcrispa, Plasmodium falciparum

---

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

Jurnal Kedokteran Brawijaya 28 (2) 2014 : 91-96

Penerbit

: .,

Deskripsi Fisik

6p

Bahasa

ISBN/ISSN

-

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

-

Info Detail Spesifik

-

Pernyataan Tanggungjawab

-

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar