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Efek Pemberian Ekstrak Kulit Jeruk Keprok (Citrus Reticulata) terhadap Cell-Cycle GI Arrest dan Apoptosis pada Sel Kultur Retinoblastoma (Citrus Peel Extract (Citrus reticulata) Effect on Cell-Cycle G1 Arrest and Apoptosis in Retinoblastoma Cell Culture)

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ABSTRACT

Retinoblastoma, an intraocular cancer occurring in early childhood, is caused by disruption in the tumor suppressor RB gene and con occur either hereditary or nonhereditary. The management involves enucleation, exenterotion, chemotherapy, laser photocoagulation, cryotherapy and radiotherapy. If left untreated, almost all patients experience intracronial extension and disseminated disease. Chemotherapeuticagents ore usually nonselective because these agents also damage normal cells in normal tissues. Therefore, the researchers search an alternative therapy such as citrus peel extract that has function as anti-carcinogenic, anti-tumor, anti-invasive, and anti-metastasis. This study is an experimental study on retinoblastoma cell cultures that are exposed to citrus peel extract. The retinoblastoma cell culture was divided in 4 groups, namely control group, cell culture + 10mg/ml citrus peel extract, cell culture + 20mg/ml citrus peel extract, and cell culture + 40mg/ml citrus peel extract. All groups were incubated for 72 hours and propidium iodide staining was performed for flowcytometry analysis.

The examination results through flowcytometry were histogram of cell cycle and apoptosis. Retinoblastoma cell culture in G1/S phose, control group (15,03±3,03), 10mg/ml citrus peel extract (12,4712,51), 20mg/ml citrus peel extract (12,30±1,33) and 40mg/ml citrus peel extract (10,80±1,52) with significant value 0,027 (p < CLOS) show that citrus peel extract do not induce GI arrest. On apoptosis data, control group (35,0±3,96), 10mg/ml citrus peel extract (33,63±0,88), 20mg/ml citrus peel extract (38,48±8,96) and 40mg/m1 citrus peel extract (64,42±2,09)(p=0,000)show that citrus peel extract can induce apoptosis in retinoblostomo cell culture. Citrus peel (Citrus reticulated consist offiavonoids that act as anti-carcinogenic, anti-tumor, anti-invasive and anti-metastasis. Citrus peel extract do not induce of cell-cycle G1 arrest but induce apoptosis process.

Keywords: Apoptosis, cell-cycle 61 arrest, citrus peel extract (Citrus reticulata), human retinoblastoma cell culture

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Jurnal Kedokteran Brawijaya 28 (2) 2014 : 68-73

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Malang : Jurnal Kedokteran Brawijaya., 2014

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6p

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0216-9347

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