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Produksi Parthenogenetik Blastosis Mencit sebagai Sumber Stem Cell

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Embryonic Stem Cells (ESCs) is a one of source for pluripotent stem cell that capable to differentiate into various cell types. This has opened up opportunities utilization of embryos as a source of stem cells. However, the use of embryos as objects of research still has ethical constraints.

Currently parthenogenetic embryo in the blastocyst stage is considered to be one of the alternative sources of ESC are "ethical" because its obtained not from the fertilization of the oocyte and sperm. Parthenogenetic embryo derived from oocyte that activation in vitro to obtain embryos in the blastocyst stage.

This research aims to produce blastocysts parthenogenetic mice as a source of ESC. In this study super ovulation performed on Swiss Webster female mice to obtain oocytes. Activation of mouse oocytes done by culturing oocytes in medium with 10 mM strontium chloride (SrCl2) and 5 mg / ml cytokalasin B for 6 hours and cultured for 4-5 days to get the embryo parthenogenetic.

The results showed that development of mice oocyte activated to be 2 pronucleus (2PN), cleavage, morula and blastocyst sequence was 65%, 97%, 90% and 23%. The conclusion of this study indicate that the parthenogenetic embryos as sources of stem cells can be produced in vitro.

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Series Title

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Call Number

Jurnal Biotek Medisiana Indonesia, 2(1)2013: 1-8

Publisher

Jakarta : Pusat Biomedis dan Teknologi Dasar Kesehatan., 2013

Collation

Jurnal Biotek Medisiana Indonesia

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ISBN/ISSN

2301-5810

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Subject(s)

blastocyst
embryonic stem cells

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