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Variasi Konsentrasi Sari Jeruk Nipis Sebagai Disinfektan Kuman Udara di Ruang Perawatan Rumah Sakit PKU Muhammadiyah Yogyakarta
ABSTRACT
The air quality in caring rooms of hospitals is one of important things that have to be considered regarding to the prevention of nosocomiat infection. The examination results of air bacterial num¬ber in the caring room of PKU Muhammadiyah Hospital of Bantu! in 11 February 2013, still ex¬ceeded the maximum threshold permitted by the regulation, i.e. 200-500 CFUlm3. One of the alternatives for controlling the condition is by using lime juice concentration as disinfectant, be¬cause the fruits contain flavonoid, an anti-inflammatory, anti-bacterial, and anti-fungal substance; citric acid, as cleansing agent; and has acidity which suitable for bacterial controlling. The pur¬pose of the study was to determine the influence of five concentration variations of lime juice in decreasing air bacterial number in the caring rooms of the hospital, by conducting a true expe¬riment which employed one group pre-test post-lest only design. The sample of air bacteria were obtained before and after the fogging process by using air pump sampler and midget impinger. The results showed that the highest the concentration. of lime juice, the highest the bacteria number will be reduced. The average reduction of air bacterial number for lime juice concen¬trations of 20%, 40%, 60%, 80% and 100% were measured 11,51%; 13,84%; 22,70%; 45,26%; and 55,20%, respectively. One way anova test acquired p-value less than 0,001, which can be cloncluded that the difference of the reduction for each concentration were statistically signifi¬cant The subsequent LSD test confirmed the evidence that 100% concentration gave the high¬est results. However, since the yielded bacterial number was still exceeding the permitted limit, it is advised that in its application, the fogging of 100% lime juice concentration should be carried out in five consecutive replications.
Keywords : caring room disinfection, lime juice fogging, air bacterial number
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