Trans Fatty Acids Increase Nitric Oxide Levels And Pancreatic Beta-Cell Necrosis In Rats (Asam lemak trans meningkatkan kadar oksida nitrit dan neksrosis sel beta pancreas pada tikus) | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Trans Fatty Acids Increase Nitric Oxide Levels And Pancreatic Beta-Cell Necrosis In Rats (Asam lemak trans meningkatkan kadar oksida nitrit dan neksrosis sel beta pancreas pada tikus)

Kusmiyati Tjahjono DK - Personal Name; Santoso - Personal Name; Dwi Ngestiningsih - Personal Name;

BACKGROUND
The prevalence of diabetes in lndonesia is increasing due to various factors, including life style changes such as trans fatty acid (TFA) intake. High TFA intake is known to be related to blood lipid profile changes resulting in cardiovascular disorders. This study was to identify the effect ofTFA on nitric oxide (NO) production and on necrosis of pancreatic beta cells.

METHODS
A study of randomized pre-test post-test design with control group. Thirty Sprague Dawley rats were divided into 3 groups, i.e. group K(control), group PI receiving a diet with 5% TFA, and P2 receiving 10% TFA. The intervention was performed for 8 weeks, NO level and pancreatic beta-cell necrosis were analyzed using Pearson's chi square test.

RESLJLTS
After 4 weeks of treatment there was no change in NO levels in group K, but increased NO in P2 (2.6-3.8 iM). At 8 weeks after treatment, NO levels in groups PI and P2 increased to 2.6-3.4 iM and 4.2-14.3 iM, respectively, while in group K only 2 rats had increased NO levels of 2.8-2.9 iM. With Pearsons chi-square test, there was a signifant difference in the proportions of necrotic pancreatic beta cells after 4 weeks and 8 weeks (p=0.000). No necrosis of beta cells was round in group K, mild necrosis in group PI (1-25%) and moderate necrosis in group P2 (26-50%).

CONCLLJSION
TFA consumption significantly increases NO levels in Sprague Daw ley rats and also results in moderate grades of necrosis of pancreatic beta cells.

Keywords : Trans fatty acids, nitric oxide levels, necrosis of pancreatic beta cells

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Location name is not set UNIVERSA MEDICINA, 32 (1) 2013: 51-59

A0004033

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Series Title: -
Call Number: UNIVERSA MEDICINA, 32 (1) 2013: 51-59
Publisher: Jakarta : FK UNIVERSITAS TRISAKTI., 2013
Collation: 9p
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ISBN/ISSN: 1907-3062
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