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Geraniin supplementation increases human keratinocyte proliferation in serum-free culture (Suplementasi geraniin meningkatkan proliferasi kultur keratinosit manusia bebas serum)

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BACKGROUND:
Various products used in cellular therapy utilize tissue culture techniques requiring keratinocyte culture. An efficient and clinically acceptable keratinocyte culture system requires supplernents with mitogenic activity. Geraniin is a phytochemical with the potential as a supplement for expansion culture of keratinocytes. The objective of the present study was to verify the mitogenic activity of geraniin on human keratinocytes

METHODS
This was an experimental study using two samples of human foreskin obtained by circurncision of a male chi Id. Epidermal keratinocytes were isolated from the foreskin samples and were divided into paired groups, comprising intervention and control groups. The intervention groups were cultured with geraniin supplementation, whereas the control groups with standard supplements, wirhout the addition of geraniin. Mitochondrial activity of the cells was evaluated by means of the 3-[4,5-dimethylthiazoI-2-yI]-2.5-diphenyl-tetrazolium-bromide (MTT) proJiferation assay. Absorbance values in each of the groups was measured at 450 nm. Data analysis was performed with the paired t-test.

RESULTS
Geraniin supplementation significantly increased the keratinocyte proliferation rares at dosages of 0.8 to 3.1 µM. An increase of 57% in the proliferation rate was obtained at a dosage of 1.6 µM, whi Ie at adosage of 12.5 µM toxic effects were starting to appear. Geraniin presumably causes increased cellular energy staru-. resulting in increased proliferation rates,

CONCLUSJON
The findings in this study provide evidence in support of the utilizarion of geraniin as a supplement for expansion culture ofkeratinocytes. Further studies may presumably identify the molecules acting as geraniin receptors and the intracellular mechanisrns underly ing the increase in proliferation rates,

Key words: Keratinocyte, geraniin, foreskin, proliferation

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UNIVERSA MEDICINA, 32 (1) 2013: 3-10

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Jakarta : FK UNIVERSITAS TRISAKTI., 2013

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8p

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1907-3062

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