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Efikasi Bacillus Thuringiensis 2 Isolat Serotipe H-10 Galur Lokal Terhadap Jentik Nyamuk Aedes Aegypti dan Anopheles Aconitus (Efficacy Two Lsolates Of Bacillus Thuringiensis Local Strains Towards Aedes Aegypti And Anopheles Aconltus Larvae)

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Bacillus thuringlensis serotype H-10 known to control the mosquito larvae (Diptera) based on type of crystal protein toxin produced. The research objective was to determine the efficacy of two isolates (KT isolates and PS isolates B. thuringiensis serotype H-10 local strains against of Aedes aegypti mosquito larvae and Anopheles aconitus. To obtain B. thuringiensis H-10 conducted by the isolation, identification and serologic testing of the soil habitat conducted in the laboratory of IVRCRD Salatiga and Institute of Biotechnology Bogor. Isolates KT at a concentration of 148.95 ppm and 341.21 ppm could kill larvae of Ae. aegypti, respectivelyfor 50% and 90%. The concentration ol49.44 ppm and 147.07 ppm can killlarvae An. aconitus respectively by 50% and 90%. Isolates of B. thuringiensis H-10 (isolates PS) at a concentration of 159.98 ppm and 341.91 ppm could kill larvac of Ae. aegypti, respectively for 50% and 90%. While the concentration of 50.84 ppm and 141.46 ppm can kill larvae An. aconitus respectively by 50% and 90%. Total Viable Spore Count (TVSC) B. thuringiensis H-10 isolates KT and PS respectively of 10.7 x 107 cells/mI and 9.3 x 107cells/ml. Each isolates has a number of spores was 10.5 x 107 spores / ml and 10.1x107 spores / mI. The results of residual toxicity isolate B. thuringiensis H-10 (KT isolates) with a concentration of 341.21 ppm and B. thuringiensis H-10 (PS isolate) with a concentration of 341.91 ppm against the larvae of Ae. aegypti instar III shows the percentage of mortality was greater than 70%, respectively on day 5 and 4. While the residual toxicity of isoZated B. thuringiensis H-10 (KT isolates) with a concentration of 147.07 ppm and PS isolates with 141.46 ppm concentration against larvae An. aconitus instar III shows the percentage of mortality was greater than 70%, respectively on day 4. The investigation shoud be developed further for form ula tion liquid and powder the local strain of B. thuringiensis H-10.

Keywords: B. thuringiensis H-10, the local strain, Ae. aegypti, An. aconitus

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VEKTORA: Jurnal vektor dan Reservoir Penyakit, 5 (

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Salatiga : Jurnal vektor dan Reservoir Penyakit., 2013

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5p

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2085-868x

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