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Konsentrasi Protein Antigen Ekskretori-Sekretori Fasciola Giqantica Asal Sapi Lokal Kabupaten Sigi, Sulawesi Tengah (Protein Concentration of Bovine Antigen Excretory-Secretory Fasciola gigantica from Local Cattle in Sigi District, Central Sulawesi)/-- Jurnal Vektor Penyakit 7 (1) 2013: 30-35

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Fasciolosis is a parasitic disease tha t ca used by liver fluke worm from Trematode class and Fasciola genus with two important species, Fasciola hepatica and Fasciola gigantica (F.gigantica). Early diagnosis offascioiosis can be detected by ELJSA method to recognize antibody used antigen capture or known as coproantiqen. Concentmtions test on protein antigen (Ag) exretory-secretory (ES) of F. gigantica was conducted in September 2010. Antigen ES used in this test, is an AgES which had been produced in previous research. ES antiqens can be used as a material for praducinq antibodies and as an inqredient in the EL/SA test. The purpose of this study was to determine the protein concentration of bavine AgESFg taken from local cattle in Sigi district. The study was un observational study with a cross-sectionul study design. Samples of infected catile liver were ran domly taken from the Sigi Biromaru abattoir (slaughterhouse). ESFg antiqens produced by collectinq worms F. gigantica from Sigi local cattle and then isolated and incubated using RPMI culture medium for four haurs at 370C. ESFg antigens characterized using Bradford method. Characterization produced AgES from three times collections of worms F. gigantica with obtained concentration were 0430, 0459, 0699 mq/ml with in an averaqe of 0.18 mg/worm. Therefore, it can be concluded that the protein concentration were 0,430- 0,699 mq/ml AgESFg taken from local cattle in Sigi district can be used as a material for producing antibodies and asan ingredient in the EUSA test.

Keywords : Fasciolosis, Excretory-secretory antigen, Fasciola gigantica

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Informasi Detail

Judul Seri

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No. Panggil

Jurnal Vektor Penyakit, 7 (1) 2013: 30-35

Penerbit

: ., 2013

Deskripsi Fisik

6p

Bahasa

ISBN/ISSN

1978-3647

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Tipe Media

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