Potensi Gen Dtx dan dtxR sebagai Marker untuk Deteksi dan Pemeriksaan Toksigenisitas Corynebacterium diphtheriae | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Potensi Gen Dtx dan dtxR sebagai Marker untuk Deteksi dan Pemeriksaan Toksigenisitas Corynebacterium diphtheriae

Sunarno - Personal Name; , Kambang Sariadji - Personal Name; Holly Arif Wibowo - Personal Name;

Corynebacterium diphtheriae is the causative agent of diphtheria. The main virulence determinant of the bacteria is diphtheria toxin, the cause of the systemic complication seen with diphtheria. Production of diphtheria toxin by toxigenic strain encoded by dtx/tox gene and repressed by dtxR gene. Gold standard for bacterial toxigenicity test carried out by conventional methods (Elek test, Guinea pig and vero cell cytotoxicity). However, Elek test have variety result, time consume and problem of the reagent availability. On the other hand, the animal (Guinea pig) testing was opposed by many animal lovers and the vero cell cytotoxicity test require high cost. The study purposed to evaluate the using of dtx and dtxR genes as a detection marker of C.diphtheriae and bacterial toxigenicity test simultaneusly by Multiplex PCR. The study examined 44 bacterial and fungal isolates, included 22 C.diphtheriae (4 reference strains and 18 clinical isolates), 5 other specieses of Corynebacterium (reference strains) and 17 non-Corynebacterium (10 reference strains and 7 stock cultures ). All of sample were examined by Multiplex PCR for 2 primer pairs targeted dtx and dtxR genes. The study showed that the Multiplex PCR for dtx and dtxR as target genes able to detect all of sample correctly thus concluded that dtx and dtxR genes could be used as a marker for alternative detection and toxigenicity test of C.diphtheriae by Multiplex PCR rapidly and accuratelly.

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Location name is not set Buletin Penelitian Kesehatan, 41 (1) 2013 : 1-10

A0003705

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Series Title: -
Call Number: Buletin Penelitian Kesehatan, 41 (1) 2013 : 1-10
Publisher: Jakarta : Puslitbang Ekologi Kesehatan,Badan Litbangkes., 2013
Collation: 10p
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ISBN/ISSN: 0125-9695
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Edition: -
Subject(s): diphtheria
GENES
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