Text

The Phytochemical Screening and Thin Layer Chromatography Results of Jatropha Gossypiifolia Seeds

---

Background:Schistosomiasis is still a health problem in Central Sulawesi. Snail Oncomelania hupensis lindoensis, the intermediary for schistosomiasis is widespread in this region. Eradication has been done by spraying chemical molluscicides. This study aimed to identify the class of chemical compounds in the methanol extract of red castor seed.

Methods: The study was conducted in May 2009. Red castor seeds were collected from Palu, Central Sulawesi. Red castor seeds extraction was done by percolation method using methanol solvent. Phytochemical screening test was performed with a tube to detect the compound in red castor bean extract. Screening was followed by thin layer chromatography testing to ensure the screening results of the test tube.

Results:Extracts that was produced from 500 grams of red castor dry seed powder with 2500 ml of methanol solvent was 250 ml thick reddish brown fluid. Phytochemical screening with a test tube showed positive results of alkaloid by the formation of deposits in Meyer test, Wagner test, and Dragendorff test. Screening the methanol extracts of red castor seed also showed positive results on saponins by foam test and LiebermanBurchard test. Positive results on Killiani Keller tests and Kedde test suggests that red castor bean extract contains cardenoline and bufadienol. Thin-layer chromatography analysis showed that the red castor bean extract is positive for terpenes with the formation of spots on the silica gel plate when terpenes was sprayed (cerium sulfate reagent).

Conclusion: Chemical components contained in the methanol extract of red castor beans consisted of alkaloids, saponins, cardenolin, bufadienol, and terpenes.

---

Availability

Detail Information

Series Title

-

Call Number

Health Science Journal of Indonesia, 3 (2) 2012: 9

Publisher

Jakarta : Sekretariat Badan Litbangkes., 2012

Collation

5p.

Language

English

ISBN/ISSN

2085-4102

Classification

-

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

SCHISTOSOMIASIS

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment