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Comparison of Rotavirus Detection from Rectal Swab and Feces in Patients with Diarrhea Symptoms

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Background: Rotavirus is the most common etiologic agent causes severe diarrhea in infants and children worldwide. Globally, every year 600,000 deaths in children less than 5 years associated with rotavirus infection. Commonly used to detect rotavirus stool samples, but getting stool samples were more difficult than rectal swab. The purpose of this study was to compare the results of the use of specimen of rectal swabs and feces to detect Rotavirus by RT-PCR method.

Methods: To evaluate rotavirus that cause diarrhea we used rectal swabs and stool samples taken from infants and identified by RT-PCR method. Data were analyzed with sensitivity and specificity analysis tests.

Results:A number 189 specimen pairs were included of which 24 were negative in both specimen types and 112 were positive in both specimen types. Forty four 42 pairs was positive in the stool specimen only and 11 pairs was positive in the rectal swab specimen only. Sensitivity of rectal swab specimen was 72.7% and specificity was 68.6%. Rectal swab positive predictive value of 91.1%, while a negative predictive value of 36.3%.

Conclusion:The result of the sensitivity and specificity of rectal swab specimen was adequate compared with the feces specimen. (Health Science Indones 2012;2:xx-xx)

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Detail Information

Series Title

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Call Number

Health Science Journal of Indonesia, 3 (2) 2012: 9

Publisher

Jakarta : Sekretariat Badan Litbangkes., 2012

Collation

4p.

Language

English

ISBN/ISSN

2087-7021

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

diarrhea
PATIENTS
ROTAVIRUS

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