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Genetic Dynamic Analysis of the H5N1 Avian Influenza Virus NS1 Gene Isolated in Bali

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Background:H5N1 Avian Influenza virus is expected to continue to mutate, potentially increasing the capacity to jump the species barrier, and can be easily transmitted between humans. This study aimed to analyze the genetic dynamics of the NS1 gene and to recognize markers of virulence in VAI H5N1 NS1 gene sequences from Balinese poultry.

Methods:The method used was isolation of RNA, NS1 gene amplification by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), electrophoresis and sequencing. Data sequence Avian influenza H5N1 virus isolates from Bali is then compared with the multiple alignment with other Indonesian isolates from different hosts accessed through 2005-2007 Gen Bank, and constructing a phylogenetic tree.

Results:The four test isolates had substitutions and deletions P42S 5 amino acids at positions 80-84 resulting in the potential for increased virulence of the virus. But no D92E, F103L and M106I substitution were found. Phylogenetic analysis showed four test isolates have a closer genetic kinship with cats and human origin isolates. Compared to the 2005 Bali isolate, the test isolates had increased nucleotide and amino acid substitutions.

Conclusions:Avian influenza virus H5N1 isolates from Bali has dynamic genetic and virulence marker had found on NS1 gene sequence. VAI H5N1 isolates from Bali underwent genetic dynamics. Virulence markers were found in the NS1 gene sequences. (Health Science Indones 2012;2:xx-xx)

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Series Title

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Call Number

Health Science Journal of Indonesia, 3 (2) 2012: 8

Publisher

Jakarta : Sekretariat Badan Litbangkes., 2012

Collation

5p.

Language

ISBN/ISSN

2087-7021

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Edition

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Subject(s)

genetics
INFLUENZA A VIRUS, H5NI SUBTYPE

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